A New Enzyme-Linked Immunosorbent Assay (ELISA) for Vi Antigen as a Potential Diagnostic for Infections by Salmonella Typhi
نویسندگان
چکیده
2053. FilmArray® Gastrointestinal (GI) Panel for Viral Acute Gastroenteritis Detection in Pediatric Patients Neena Kanwar, PhD; Jami Jackson, DO; Susan Duffy, MD; Kimberle Chapin, MD; Daniel Cohen, MD; Amy Leber, PhD; Judy a. Daly, PhD; Andrew Pavia, MD, FIDSA, FSHEA, FPIDS; Chari Larsen, MD; Tanya Baca, MD; Jeffery Bender, MD; Jennifer Dien Bard, Ph.D; Ara Festekjian, MD, MS; Kristen Holmberg, MS; Kevin Bourzac, PhD and Rangaraj Selvarangan, PhD; Children’s Mercy Hospital, Kansas City, Missouri, Rhode Island Hospital, Providence, RI, Pathology, Rhode Island, Hasbro Medical Center, Providence, Rhode Island, Pediatrics, Section of Emergency Medicine, Nationwide Children’s Hospital, Columbus, Ohio, Department of Laboratory Medicine, Nationwide Children’s Hospital, Columbus, Ohio, Department of Pathology, Division of Clinical Pathology, University of Utah School of Medicine, Salt Lake City, Utah, Department of Pediatrics, Division of Pediatric Infectious Diseases, University of Utah School of Medicine, Salt Lake City, Utah, Pediatrics, University of Utah, Salt Lake City, Utah, University of Utah, Salt Lake City, Utah, Pediatrics, Children’s Hospital Los Angeles, Los Angeles, California, Pathology and Laboratory Medicine, Children’s Hospital Los Angeles, Los Angeles, California, Children’s Hospital Los Angeles, Los Angeles, California, BioFire Diagnostics, LLC, Salt Lake City, Utah, Children’s Mercy Hospital and Clinics, Kansas City, Missouri
منابع مشابه
Development and evaluation of an enzyme-linked immunosorbent assay for serum Vi antibodies for detection of chronic Salmonella typhi carriers.
An enzyme-linked immunosorbent assay (ELISA) measuring, in serum, immunoglobulin G (IgG), IgM, and IgA to Vi capsular polysaccharide antigen that was tyraminated (Vi-Tyr) to increase its binding efficiency to microtiter plates was compared with the standard passive hemagglutination assay (PHA) as a screening test for chronic Salmonella typhi carriers. Initially, three populations were evaluated...
متن کاملDevelopment of a bead immunoassay to measure Vi polysaccharide-specific serum IgG after vaccination with the Salmonella enterica serovar Typhi Vi polysaccharide.
Vi polysaccharide from Salmonella enterica serotype Typhi is used as one of the available vaccines to prevent typhoid fever. Measurement of Vi-specific serum antibodies after vaccination with Vi polysaccharide by enzyme-linked immunosorbent assay (ELISA) may be complicated due to poor binding of the Vi polysaccharide to ELISA plates resulting in poor reproducibility of measured antibody respons...
متن کاملComparison of diffusion-in-gel enzyme-linked immunosorbent assay with conventional serological methods for detection of class-specific antibodies to Salmonella typhi O antigen.
The diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA) is a new and simple method for quantitation of antibodies, based on the ability of antibodies to diffuse from wells in gel and adsorb to antigen which is bound to a polystyrene surface. The antigen-antibody reaction is visualized with a color reaction caused by horseradish peroxidase-conjugated class-specific anti-immunoglobins....
متن کاملEnzyme-linked immunosorbent assay for detection of Salmonella typhi Vi antigen in urine from typhoid patients.
Because typhoid fever continues to be a major cause of illness in many developing countries, there is a clear need for a sensitive and specific test that will permit rapid laboratory diagnosis of the disease. An enzyme-linked immunosorbent assay (ELISA) has recently been developed and tested, both in the laboratory and in a clinical situation, for its ability to detect Vi antigen in urine. The ...
متن کاملFifth Percentile Cutoff Values for Antipneumococcal Polysaccharide and Anti-Salmonella typhi Vi IgG Describe a Normal Polysaccharide Response
BACKGROUND Serotype-specific antibody responses to unconjugated pneumococcal polysaccharide vaccine (PPV) evaluated by a World Health Organization (WHO)-standardized enzyme-linked immunosorbent assay (ELISA) are the gold standard for diagnosis of specific polysaccharide antibody deficiency (SAD). The American Academy of Allergy, Asthma and Immunology (AAAAI) has proposed guidelines to interpret...
متن کاملDetection of urinary Vi antigen as a diagnostic test for typhoid fever.
Since Vi antigen is limited primarily to Salmonella typhi, it has been thought that detection of the antigen may be a useful method for diagnosing acute typhoid fever. The slide coagglutination method and enzyme-linked immunosorbent assay have recently been suggested as ways to detect small quantities of Vi antigen in urine. In Santiago, Chile, we compared the results of these two methods in pa...
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